Abstract
Cyclophilins belong to the enzyme class of peptidyl prolyl cis-trans isomerases which catalyze the cis-trans isomerization of prolyl bonds in peptides and proteins in different folding states. Cyclophilins have been shown to be involved in a multitude of cellular functions like cell growth, proliferation, and motility. Among the 20 human cyclophilin isoenzymes, the two most abundant members of the cyclophilin family, CypA and CypB, exhibit specific cellular functions in several inflammatory diseases, cancer development, and HCV replication. A small-molecule inhibitor on the basis of aryl 1-indanylketones has now been shown to discriminate between CypA and CypB in vitro. CypA binding of this inhibitor has been characterized by fluorescence anisotropy- and isothermal titration calorimetry-based cyclosporin competition assays. Inhibition of CypA- but not CypB-mediated chemotaxis of mouse CD4(+) T cells by the inhibitor provided biological proof of discrimination in vivo.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Benzofurans / chemistry
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Binding, Competitive
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Biocatalysis
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CD4-Positive T-Lymphocytes / cytology
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Catalytic Domain
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Chemotaxis / drug effects
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Cyclophilin A / antagonists & inhibitors
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Cyclophilin A / chemistry
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Cyclophilin A / metabolism
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Cyclophilins / antagonists & inhibitors*
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Cyclophilins / chemistry*
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Cyclophilins / metabolism
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Cyclosporine / chemistry
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Cyclosporine / metabolism
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Enzyme Inhibitors / chemistry*
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Enzyme Inhibitors / pharmacology*
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Fluorescence Polarization
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Humans
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Indans / chemistry
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Indans / metabolism
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Indans / pharmacology
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Isoenzymes / antagonists & inhibitors
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Isoenzymes / chemistry
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Isoenzymes / metabolism
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Kinetics
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Mice
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Mice, Inbred Strains
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Molecular Structure
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Protein Binding
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Recombinant Proteins / antagonists & inhibitors
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Recombinant Proteins / chemistry
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Recombinant Proteins / metabolism
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Thermodynamics
Substances
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Benzofurans
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Enzyme Inhibitors
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Indans
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Isoenzymes
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Recombinant Proteins
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cyclophilin B
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Cyclosporine
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Cyclophilin A
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Cyclophilins